Suggested Procedures for the Detection and Identification of Certain Finfish and Shellfish Pathogens (Blue Book 2007 Edition), 3-year subscription
CD-ROM, 2007, updated regularly
Published by the Fish Health Section, American Fisheries Society.
Publication date: November 2007
Summary
Originally published in loose-leaf format as the Blue Book, this updated, searchable CD contains both the familiar chapters of the "Suggested Procedures for the Detection and Identification of Certain Finfish and Shellfish Pathogens" and the newly revised "Standard Procedures for Aquatic Animal Health Inspections" co-authored by the U.S. Fish and Wildlife Service and the AFS Fish Health Section. This new format allows the user to easily access information and contains numerous color photographs and video clips.
CONTENTS
Section 1
Diagnostic Procedures for Finfish and Shellfish Pathogens
Chapter 1 Bacterial Diseases of Fish
1.1 General Procedures for Bacteriology 1.2 Flow Chart for the Presumptive Identification of Selected Bacteria from Fishes GRAM-NEGATIVE BACTERIA 1.3 Bacterial Gill Disease 1.4 Coldwater Disease 1.5 Columnaris Disease 1.6 Edwardsiella tarda Septicemia 1.7 Enteric Redmouth Disease 1.8 Enteric Septicemia 1.9 Furunculosis 1.10 Other Diseases Caused by Aeromonas salmonicida 1.11 Motile Aeromonas Septicemia 1.12 Vibriosis 1.13 Coldwater Vibriosis GRAM-POSITIVE BACTERIA 1.14 Bacterial Kidney Disease 1.14.A1 Bacterial Kidney Disease Methods Appendix 1 1.14.A2 Bacterial Kidney Disease Methods Appendix 2 1.14.A3 Bacterial Kidney Disease Methods Appendix 3 1.14.A4 Bacterial Kidney Disease Methods Appendix 4 1.14.A5 Bacterial Kidney Disease Methods Appendix 5 1.15 Pseudokidney Disease 1.16 Streptococcal Disease OTHER 1.17 Piscirickettsiosis
Chapter 2 Viral Diseases of Fish
2.1 General Procedures for Virology 2.2 Channel Catfish Virus Disease 2.3 Erythrocytic Inclusion Body Syndrome 2.4 Herpesvirus Diseases of Salmonids 2.5 Infectious Hematopoietic Necrosis 2.6 Infectious Pancreatic Necrosis 2.7 Viral Erythrocytic Necrosis 2.8 Viral Hemorrhagic Septicemia 2.9 Infectious Salmon Anemia 2.9.A1 Infectious Salmon Anemia Appendix 1 2.9.A2 Infectious Salmon Anemia Appendix 2 2.10 Spring Viremia of Carp 2.11 Koi Herpesvirus Disease 2.12 Goldfish Herpesviral Hematopoietic Necrosis 2.13 Largemouth Bass Virus Disease 2.A1 Methods for Testing for the Presence of Mycoplasma in Stock Cell Cultures
Appendix 1
Chapter 3 Parasitic Diseases of Fish
3.1 General Procedures for Parasitology 3.1.A1 Key to Major Taxa of Adult Parasites of Fishes 3.2 Ichthyobodiasis 3.3 Diplomonad (Hexamitid) Flagellates: Diplomonadiasis, Hexamitosis, Spironucleosis 3.4 Ovipleistophoriasis (Pleistophora): Ovary Parasite of Golden Shiners 3.5 Salmonid Ceratomyxosis 3.5.A1 Salmonid Ceratomyxosis Methods Appendix 1 3.6 Whirling Disease of Salmonids 3.7 Proliferative Gill Disease 3.7.A1 Proliferative Gill Disease Methods Appendix 1 3.8 Proliferative Kidney Disease 3.9 Ichthyophthiriasis 3.10 External Infection by Ciliated Parasites 3.11 Monogenean Diseases 3.12 Bothriocephalosis 3.13 Lernaeid Parasitism 3.14 Gill Maggot Disease (Genus Salmincola) 3.15 Sea Lice 3.16 Branchiuran Fish Louse Disease 3.17 Centrocestiasis
Chapter 4 Mycotic Diseases of Fish
4.1 General Procedures for Mycology 4.2 Branchiomyces 4.3 Ichthyophonus Disease 4.4 Saprolegnia 4.5 Miscellaneous Mycotic Diseases
Chapter 5 Diseases of Molluscan Shellfish
5.1 Diseases of Shellfish Introduction 5.2 Perkinsus sp. Infections of Marine Molluscs 5.3 Haplosporidiosis of Oysters 5.4 Oyster Seaside Haplosporidiosis 5.5 Velar Virus Disease of Pacific Oysters 5.6 Mikrocytosis (Denman Island Disease) 5.7 Nocardiosis of Pacific Oysters 5.8 Bonamiasis of Oysters 5.9 Hexamitiasis of Oysters 5.10 Marteiliasis and Marteilioidiasis of Shellfish 5.11 Shell Disease of Oysters 5.12 Hematopoietic Neoplasia of Bivalve Molluscs 5.13 Miscellaneous Diseases of Molluscs 5.14 Juvenille Oyster Disease of Eastern Oysters
Chapter 6 Diseases of Crustaceans
6.1 Necrotizing Hepatopancreatitis Virus of Peneaid Shrimp 6.2 Taura Syndrome Virus of Peneaid Shrimp 6.3 Lagenidium of Decapod Crustaceans
Section 2
USFWS/AFS-FHS Standard Procedures for Aquatic Animal Health Inspections
Chapter 1 Introduction
1.1 Introduction
Chapter 2 Sampling
2.1 Sampling Introduction 2.2 Sampling 2.3 Reagents, Media, and Media Preparation 2.4 Glossary 2.5 References
Chapter 3 Bacteriology
3.1 Bacteriology Introduction 3.2 Aeromonas salmonicida (Furunculosis) 3.3 Yersinia ruckeri (Enteric Redmouth Disease, ERM) 3.4 Edwardsiella ictaluri (Enteric Septicemia of Catfish, ESC) 3.5 Renibacterium salmoninarum (Bacterial Kidney Disease, BKD) 3.6 Piscirickettsia salmonis 3.7 Reagents, Media, and Media Preparation 3.8 Bacterial Identification Techniques 3.9 Glossary 3.10 References 3.A1 Laboratory Reference Flow Chart Appendix 1 3.A2 Profiles Obtained with API-20E for Known Fish Pathogens 3.A3.A Worksheet A - PCR Sample Data/Log Sheet 3.A3.B Worksheet B - Initial Amplification of Nucleic Acid by PCR for the Confirmation of R. salmoninarum 3.A3.C Worksheet C - Nested Amplification of Nucleic Acid by PCR for the Confirmation of R. salmoninarum 3.A3.D Worksheet D - Initial Amplification of Nucleic Acid by PCR for the Confirmation of P. salmonis 3.A3.E Worksheet E - Nested Amplification of Nucleic Acid by PCR for the Confirmation of P. salmonis 3.A3.F Worksheet F - Direct Amplification of Nucleic Acid by PCR for the Confirmation of P. salmonis 3.A3.G Worksheet G - Photodocumentation of the PCR Product Gel
Chapter 4 Virology
4.1 Virology Introduction 4.2 Selection of Appropriate Cell Lines 4.3 Cell Culture 4.4 Sample Processing Procedures 4.5 Screening Method for Viral Isolation 4.6 Identification of Viruses 4.7 Serum Neutralization 4.8 Indirect Fluorescent Antibody (IFAT) Procedure 4.9 Reagents and Media 4.10 Glossary 4.11 References 4.A1.A Worksheet A - PCR Sample Data/Log Sheet 4.A1.B Worksheet B - Amplification of Nucleic Acid by PCR for the Confirmation of Viral Fish Pathogens 4.A1.B.1 Worksheet B.1 - Infectious Hematopoietic Necrosis Virus (IHNV) 4.A1.B.2 Worksheet B.2 - Infectious Pancreatic Necrosis Virus (IPNV) 4.A1.B.3 Worksheet B.3 - Infectious Salmon Anemia Virus (ISAV) 4.A1.B.4 Worksheet B.4 - Largemouth Bass Virus (LMBV) 4.A1.B.5 Worksheet B.5 - Oncorhynchus masou Virus (OMV) 4.A1.B.6 Worksheet B.6 - Spring Viremia of Carp Virus (SVCV) 4.A1.B.7 Worksheet B.7 - Viral Hemorrhagic Septicemia Virus (VHSV) 4.A1.C Worksheet C - Photodocumentation of the PCR Product Gel 4.A2 Cell Enumeration
Chapter 5 Parasitology
5.1 Parasitology Introduction 5.2 Myxobolus cerebralis (Whirling Disease) 5.3 Ceratomyxa shasta (Ceratomyxosis) 5.4 Tetracapsula bryosalmonae (Proliferative Kidney Disease) 5.5 Bothriocephalus acheilognathi (Bothriocephalosis, Asian Tapeworm) 5.6 Media and Reagents 5.7 Glossary 5.8 References
Chapter 6 Polymerase Chain Reaction (PCR)
6.1 Polymerase Chain Reaction (PCR) Introduction 6.2 PCR - Quality Assurance/Quality Control 6.3 PCR Protocols 6.4 Reagents 6.5 Analysis of Extracted DNA Using an UV Spectrophotometer 6.6 Table of PCR Protocols 6.7 References
Appendix 1 The Handbook and Oversight Committee
A1.1 Format of the Handbook A1.2 Handbook Revision and Oversight Committee A1.3 Process for Amending the Handbook
Appendix 2 Handbook Committee Members
A2.1 2000 - 2002 Handbook Development Committee A2.2 2002 - 2003 Handbook Development Committee A2.3 2003 - 2004 Handbook Development Committee A2.4 2004 - 2005 Handbook Development Committee A2.5 2006 - 2007 Handbook Development Committee
Appendix 3 Position Statements
A3.1 Position Statements Introduction A3.2 Sampling Position Statements A3.3 Bacteriology Position Statements A3.4 Virology Position Statements A3.5 Parasitology Position Statements A3.6 Polymerase Chain Reaction (PCR) Position Statements A3.7 Appendix 1 The Handbook and Oversight Committee Position Statements
Published by the Fish Health Section, American Fisheries Society.
Publication date: November 2007
Summary
Originally published in loose-leaf format as the Blue Book, this updated, searchable CD contains both the familiar chapters of the "Suggested Procedures for the Detection and Identification of Certain Finfish and Shellfish Pathogens" and the newly revised "Standard Procedures for Aquatic Animal Health Inspections" co-authored by the U.S. Fish and Wildlife Service and the AFS Fish Health Section. This new format allows the user to easily access information and contains numerous color photographs and video clips.
CONTENTS
Section 1
Diagnostic Procedures for Finfish and Shellfish Pathogens
Chapter 1 Bacterial Diseases of Fish
1.1 General Procedures for Bacteriology 1.2 Flow Chart for the Presumptive Identification of Selected Bacteria from Fishes GRAM-NEGATIVE BACTERIA 1.3 Bacterial Gill Disease 1.4 Coldwater Disease 1.5 Columnaris Disease 1.6 Edwardsiella tarda Septicemia 1.7 Enteric Redmouth Disease 1.8 Enteric Septicemia 1.9 Furunculosis 1.10 Other Diseases Caused by Aeromonas salmonicida 1.11 Motile Aeromonas Septicemia 1.12 Vibriosis 1.13 Coldwater Vibriosis GRAM-POSITIVE BACTERIA 1.14 Bacterial Kidney Disease 1.14.A1 Bacterial Kidney Disease Methods Appendix 1 1.14.A2 Bacterial Kidney Disease Methods Appendix 2 1.14.A3 Bacterial Kidney Disease Methods Appendix 3 1.14.A4 Bacterial Kidney Disease Methods Appendix 4 1.14.A5 Bacterial Kidney Disease Methods Appendix 5 1.15 Pseudokidney Disease 1.16 Streptococcal Disease OTHER 1.17 Piscirickettsiosis
Chapter 2 Viral Diseases of Fish
2.1 General Procedures for Virology 2.2 Channel Catfish Virus Disease 2.3 Erythrocytic Inclusion Body Syndrome 2.4 Herpesvirus Diseases of Salmonids 2.5 Infectious Hematopoietic Necrosis 2.6 Infectious Pancreatic Necrosis 2.7 Viral Erythrocytic Necrosis 2.8 Viral Hemorrhagic Septicemia 2.9 Infectious Salmon Anemia 2.9.A1 Infectious Salmon Anemia Appendix 1 2.9.A2 Infectious Salmon Anemia Appendix 2 2.10 Spring Viremia of Carp 2.11 Koi Herpesvirus Disease 2.12 Goldfish Herpesviral Hematopoietic Necrosis 2.13 Largemouth Bass Virus Disease 2.A1 Methods for Testing for the Presence of Mycoplasma in Stock Cell Cultures
Appendix 1
Chapter 3 Parasitic Diseases of Fish
3.1 General Procedures for Parasitology 3.1.A1 Key to Major Taxa of Adult Parasites of Fishes 3.2 Ichthyobodiasis 3.3 Diplomonad (Hexamitid) Flagellates: Diplomonadiasis, Hexamitosis, Spironucleosis 3.4 Ovipleistophoriasis (Pleistophora): Ovary Parasite of Golden Shiners 3.5 Salmonid Ceratomyxosis 3.5.A1 Salmonid Ceratomyxosis Methods Appendix 1 3.6 Whirling Disease of Salmonids 3.7 Proliferative Gill Disease 3.7.A1 Proliferative Gill Disease Methods Appendix 1 3.8 Proliferative Kidney Disease 3.9 Ichthyophthiriasis 3.10 External Infection by Ciliated Parasites 3.11 Monogenean Diseases 3.12 Bothriocephalosis 3.13 Lernaeid Parasitism 3.14 Gill Maggot Disease (Genus Salmincola) 3.15 Sea Lice 3.16 Branchiuran Fish Louse Disease 3.17 Centrocestiasis
Chapter 4 Mycotic Diseases of Fish
4.1 General Procedures for Mycology 4.2 Branchiomyces 4.3 Ichthyophonus Disease 4.4 Saprolegnia 4.5 Miscellaneous Mycotic Diseases
Chapter 5 Diseases of Molluscan Shellfish
5.1 Diseases of Shellfish Introduction 5.2 Perkinsus sp. Infections of Marine Molluscs 5.3 Haplosporidiosis of Oysters 5.4 Oyster Seaside Haplosporidiosis 5.5 Velar Virus Disease of Pacific Oysters 5.6 Mikrocytosis (Denman Island Disease) 5.7 Nocardiosis of Pacific Oysters 5.8 Bonamiasis of Oysters 5.9 Hexamitiasis of Oysters 5.10 Marteiliasis and Marteilioidiasis of Shellfish 5.11 Shell Disease of Oysters 5.12 Hematopoietic Neoplasia of Bivalve Molluscs 5.13 Miscellaneous Diseases of Molluscs 5.14 Juvenille Oyster Disease of Eastern Oysters
Chapter 6 Diseases of Crustaceans
6.1 Necrotizing Hepatopancreatitis Virus of Peneaid Shrimp 6.2 Taura Syndrome Virus of Peneaid Shrimp 6.3 Lagenidium of Decapod Crustaceans
Section 2
USFWS/AFS-FHS Standard Procedures for Aquatic Animal Health Inspections
Chapter 1 Introduction
1.1 Introduction
Chapter 2 Sampling
2.1 Sampling Introduction 2.2 Sampling 2.3 Reagents, Media, and Media Preparation 2.4 Glossary 2.5 References
Chapter 3 Bacteriology
3.1 Bacteriology Introduction 3.2 Aeromonas salmonicida (Furunculosis) 3.3 Yersinia ruckeri (Enteric Redmouth Disease, ERM) 3.4 Edwardsiella ictaluri (Enteric Septicemia of Catfish, ESC) 3.5 Renibacterium salmoninarum (Bacterial Kidney Disease, BKD) 3.6 Piscirickettsia salmonis 3.7 Reagents, Media, and Media Preparation 3.8 Bacterial Identification Techniques 3.9 Glossary 3.10 References 3.A1 Laboratory Reference Flow Chart Appendix 1 3.A2 Profiles Obtained with API-20E for Known Fish Pathogens 3.A3.A Worksheet A - PCR Sample Data/Log Sheet 3.A3.B Worksheet B - Initial Amplification of Nucleic Acid by PCR for the Confirmation of R. salmoninarum 3.A3.C Worksheet C - Nested Amplification of Nucleic Acid by PCR for the Confirmation of R. salmoninarum 3.A3.D Worksheet D - Initial Amplification of Nucleic Acid by PCR for the Confirmation of P. salmonis 3.A3.E Worksheet E - Nested Amplification of Nucleic Acid by PCR for the Confirmation of P. salmonis 3.A3.F Worksheet F - Direct Amplification of Nucleic Acid by PCR for the Confirmation of P. salmonis 3.A3.G Worksheet G - Photodocumentation of the PCR Product Gel
Chapter 4 Virology
4.1 Virology Introduction 4.2 Selection of Appropriate Cell Lines 4.3 Cell Culture 4.4 Sample Processing Procedures 4.5 Screening Method for Viral Isolation 4.6 Identification of Viruses 4.7 Serum Neutralization 4.8 Indirect Fluorescent Antibody (IFAT) Procedure 4.9 Reagents and Media 4.10 Glossary 4.11 References 4.A1.A Worksheet A - PCR Sample Data/Log Sheet 4.A1.B Worksheet B - Amplification of Nucleic Acid by PCR for the Confirmation of Viral Fish Pathogens 4.A1.B.1 Worksheet B.1 - Infectious Hematopoietic Necrosis Virus (IHNV) 4.A1.B.2 Worksheet B.2 - Infectious Pancreatic Necrosis Virus (IPNV) 4.A1.B.3 Worksheet B.3 - Infectious Salmon Anemia Virus (ISAV) 4.A1.B.4 Worksheet B.4 - Largemouth Bass Virus (LMBV) 4.A1.B.5 Worksheet B.5 - Oncorhynchus masou Virus (OMV) 4.A1.B.6 Worksheet B.6 - Spring Viremia of Carp Virus (SVCV) 4.A1.B.7 Worksheet B.7 - Viral Hemorrhagic Septicemia Virus (VHSV) 4.A1.C Worksheet C - Photodocumentation of the PCR Product Gel 4.A2 Cell Enumeration
Chapter 5 Parasitology
5.1 Parasitology Introduction 5.2 Myxobolus cerebralis (Whirling Disease) 5.3 Ceratomyxa shasta (Ceratomyxosis) 5.4 Tetracapsula bryosalmonae (Proliferative Kidney Disease) 5.5 Bothriocephalus acheilognathi (Bothriocephalosis, Asian Tapeworm) 5.6 Media and Reagents 5.7 Glossary 5.8 References
Chapter 6 Polymerase Chain Reaction (PCR)
6.1 Polymerase Chain Reaction (PCR) Introduction 6.2 PCR - Quality Assurance/Quality Control 6.3 PCR Protocols 6.4 Reagents 6.5 Analysis of Extracted DNA Using an UV Spectrophotometer 6.6 Table of PCR Protocols 6.7 References
Appendix 1 The Handbook and Oversight Committee
A1.1 Format of the Handbook A1.2 Handbook Revision and Oversight Committee A1.3 Process for Amending the Handbook
Appendix 2 Handbook Committee Members
A2.1 2000 - 2002 Handbook Development Committee A2.2 2002 - 2003 Handbook Development Committee A2.3 2003 - 2004 Handbook Development Committee A2.4 2004 - 2005 Handbook Development Committee A2.5 2006 - 2007 Handbook Development Committee
Appendix 3 Position Statements
A3.1 Position Statements Introduction A3.2 Sampling Position Statements A3.3 Bacteriology Position Statements A3.4 Virology Position Statements A3.5 Parasitology Position Statements A3.6 Polymerase Chain Reaction (PCR) Position Statements A3.7 Appendix 1 The Handbook and Oversight Committee Position Statements
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If you have a question about one of our titles please contact: Book Department | American Fisheries Society | 5410 Grosvenor Lane Suite 110 | Bethesda, MD 20814-2199 | Phone: 301-897-8616, ext. 231 | Fax: 301-897-5080 | E-mail: alerner@fisheries.org
If you prefer not to order online, please contact our orders department (Books International) to place your order. | Phone: 703-661-1570 | Fax: 703-996-1010 | Email: bimail@presswarehouse.com | Mail: American Fisheries Society | c/o Books International | P.O. Box 605 | Herndon, VA 20172
